This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0041326
PrimePCR™ PreAmp for SYBR® Green Assay: BRCA1, Human
PrimePCR™ Template for SYBR® Green Assay: BRCA1, Human
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors DNA damage sensors and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II and through the C-terminal domain also interacts with histone deacetylase complexes. This protein thus plays a role in transcription DNA repair of double-stranded breaks and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants some of which are disease-associated mutations have been described for this gene but the full-length natures of only some of these variants has been described. A related pseudogene which is also located on chromosome 17 has been identified. [provided by RefSeq May 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.