PrimePCR™ SYBR® Green Assay: TTC4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041309

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038587
Assay Design:   exonic
Chromosome Location:   1:55208082-55208181question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000371281

Gene Information

This gene encodes a protein that contains tetratricopeptide (TPR) repeats. The 34-amino acid tetratricopeptide repeat motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity.[provided by RefSeq Jun 2011]

Gene Symbol:   TTC4
Gene Name:   tetratricopeptide repeat domain 4
Aliases:   DKFZp781B0622, FLJ41930, MGC5097
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000243725
Entrez:   7268
UniGene:   Hs.729029
Chromosome Mapping:   1p32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.330000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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