PrimePCR™ SYBR® Green Assay: MAFA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041282

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038560
Assay Design:   exonic
Chromosome Location:   8:144510727-144510916question
Amplicon Length:   160
Splice Variants Targeted:   ENST00000333480

Gene Information

MAFA is a transcription factor that binds RIPE3b a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al. 2002 [PubMed 12011435]).[supplied by OMIM Mar 2008]

Gene Symbol:   MAFA
Gene Name:   v-maf musculoaponeurotic fibrosarcoma oncogene homolog A (avian)
Aliases:   RIPE3b1, hMafA
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000182759
Entrez:   389692
UniGene:   Hs.521914
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 36.310000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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