PrimePCR™ SYBR® Green Assay: KCNIP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041251

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038529
Assay Design:   exonic
Chromosome Location:   10:103587646-103587748question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000348850 ENST00000356640 ENST00000370046 ENST00000434163 ENST00000353068 ENST00000461105 ENST00000343195 ENST00000239117 ENST00000358038

Gene Information

This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs) which belongs to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents and hence neuronal excitability in response to changes in intracellular calcium. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified from this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   KCNIP2
Gene Name:   Kv channel interacting protein 2
Aliases:   DKFZp566L1246, KCHIP2, MGC17241
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000120049
Entrez:   30819
UniGene:   Hs.97044
Chromosome Mapping:   10q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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