PrimePCR™ SYBR® Green Assay: GSPT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041240

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038518
Assay Design:   exonic
Chromosome Location:   16:11990494-11990626question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000434724 ENST00000439887 ENST00000565267 ENST00000563468 ENST00000420576 ENST00000568849 ENST00000562169

Gene Information

Description Not Available

Gene Symbol:   GSPT1
Gene Name:   G1 to S phase transition 1
Aliases:   551G9.2, ETF3A, FLJ38048, FLJ39067, GST1, eRF3a
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103342
Entrez:   2935
Chromosome Mapping:   16p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 36.020000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download