PrimePCR™ SYBR® Green Assay: LPIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041229

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LPIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LPIN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038507
Assay Design:   exonic
Chromosome Location:   2:11905659-11905756question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000425416 ENST00000449576 ENST00000423495 ENST00000441684 ENST00000256720 ENST00000396099 ENST00000396098

Gene Information

This gene represents a candidate gene for human lipodystrophy characterized by loss of body fat fatty liver hypertriglyceridemia and insulin resistance. Mouse studies suggest that this gene functions during normal adipose tissue development and may also play a role in human triglyceride metabolism. [provided by RefSeq Jul 2008]

Gene Symbol:   LPIN1
Gene Name:   lipin 1
Aliases:   DKFZp781P1796, KIAA0188, PAP1
RefSeq:   NC_000002.11 NG_012843.1 NT_005334.16
Ensembl:   ENSG00000134324
Entrez:   23175
Chromosome Mapping:   2p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download