PrimePCR™ SYBR® Green Assay: ANO10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041196

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038474
Assay Design:   exonic
Chromosome Location:   3:43647250-43647352question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000292246 ENST00000350459 ENST00000396091 ENST00000414522 ENST00000451430 ENST00000427171 ENST00000428472 ENST00000444344 ENST00000456438 ENST00000413397 ENST00000439141 ENST00000428831 ENST00000436073

Gene Information

The transmembrane protein encoded by this gene is a member of a family of calcium-activated chloride channels. Defects in this gene may be a cause of autosomal recessive spinocerebellar ataxia-10. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2011]

Gene Symbol:   ANO10
Gene Name:   anoctamin 10
Aliases:   FLJ10375, MGC47890, SCAR10, TMEM16K
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000160746
Entrez:   55129
UniGene:   Hs.656657
Chromosome Mapping:   3p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download