PrimePCR™ SYBR® Green Assay: LRRC24, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041176

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038454
Assay Design:   exonic
Chromosome Location:   8:145748593-145748686question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000418477 ENST00000383213 ENST00000292524 ENST00000529415 ENST00000533758 ENST00000313050 ENST00000374677 ENST00000310127 ENST00000539485 ENST00000441854 ENST00000297508 ENST00000405088 ENST00000431735 ENST00000543443 ENST00000296933 ENST00000265094 ENST00000393802 ENST00000425623 ENST00000303045 ENST00000435777 ENST00000293261 ENST00000435956 ENST00000456261 ENST00000239587 ENST00000408888 ENST00000264059

Gene Information

Description Not Available

Gene Symbol:   LRRC24
Gene Name:   leucine rich repeat containing 24
Aliases:   LRRC14OS, MGC111484
RefSeq:   NC_000008.10 NG_016430.1 NT_037704.5
Ensembl:   ENSG00000254402
Entrez:   441381
UniGene:   Hs.105685
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.610000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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