PrimePCR™ SYBR® Green Assay: AC135352.2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041170

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038448
Assay Design:   exonic
Chromosome Location:   8:12848420-12848521question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000447063 ENST00000524591 ENST00000400069 ENST00000528753 ENST00000532376 ENST00000445495

Gene Information

Description Not Available

Gene Symbol:   AC135352.2
Gene Name:   Putative methyltransferase KIAA1456
Aliases:   C8orf79, FLJ36980, MGC43113
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000250305
Entrez:   57604
UniGene:   Hs.202521
Chromosome Mapping:   8p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.200000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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