PrimePCR™ SYBR® Green Assay: TMEM110-MUSTN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041168

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMEM110-MUSTN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMEM110-MUSTN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038446
Assay Design:   exonic
Chromosome Location:   3:52867232-52867371question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000423811 ENST00000356257 ENST00000359015 ENST00000355845 ENST00000545108 ENST00000438735 ENST00000304552 ENST00000458629 ENST00000374051 ENST00000536169 ENST00000486659 ENST00000446157 ENST00000514466 ENST00000504329 ENST00000369393 ENST00000428876

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring TMEM110 (transmembrane protein 110) and MUSTN1 (musculoskeletal embryonic nuclear protein 1) genes. The read-through transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq Nov 2010]

Gene Symbol:   TMEM110-MUSTN1
Gene Name:   TMEM110-MUSTN1 readthrough
Aliases:   DKFZp667E1121
RefSeq:   Not Available
Ensembl:   ENSG00000248592
Entrez:   100526772
Chromosome Mapping:   -

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download