PrimePCR™ SYBR® Green Assay: HIST1H2AM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041141

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIST1H2AM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIST1H2AM, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038419
Assay Design:   exonic
Chromosome Location:   6:27860824-27860948question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000259791 ENST00000575379 ENST00000602637 ENST00000377791 ENST00000359193 ENST00000377459 ENST00000358739 ENST00000333151 ENST00000330180 ENST00000357320 ENST00000359611 ENST00000262715 ENST00000556935 ENST00000369161 ENST00000369159 ENST00000331380 ENST00000331128 ENST00000450042 ENST00000317977 ENST00000395647 ENST00000395643 ENST00000395645 ENST00000417251 ENST00000395642 ENST00000292616 ENST00000284548 ENST00000366709 ENST00000583968 ENST00000580456 ENST00000228929 ENST00000579360

Gene Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A H2B H3 and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes. The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq Jul 2008]

Gene Symbol:   HIST1H2AM
Gene Name:   histone cluster 1, H2am
Aliases:   H2A.1, H2A/n, H2AFN, HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, dJ193B12.1
RefSeq:   NC_000006.11 NG_000009.2 NT_007592.15
Ensembl:   ENSG00000233224
Entrez:   8336
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.990000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download