PrimePCR™ SYBR® Green Assay: CT45A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041138

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CT45A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CT45A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038416
Assay Design:   exonic
Chromosome Location:   X:134847219-134847406question
Amplicon Length:   158
Splice Variants Targeted:   ENST00000582330 ENST00000335464 ENST00000370741 ENST00000361484 ENST00000370736 ENST00000370734 ENST00000434966 ENST00000420087 ENST00000370724 ENST00000448053 ENST00000316843 ENST00000594565 ENST00000596640

Gene Information

Description Not Available

Gene Symbol:   CT45A1
Gene Name:   cancer/testis antigen family 45, member A1
Aliases:   CT45, CT45-1, CT45.1
RefSeq:   NC_000023.10 NT_011786.16
Ensembl:   ENSG00000232478
Entrez:   541466
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.230000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download