PrimePCR™ SYBR® Green Assay: TP53TG3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041070

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: TP53TG3B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TP53TG3B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038348
Assay Design:   exonic
Chromosome Location:   16:33205754-33205845question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000399012 ENST00000381657 ENST00000369784 ENST00000216962 ENST00000398664 ENST00000569631 ENST00000354614 ENST00000398682 ENST00000569420 ENST00000398680 ENST00000561509 ENST00000360260 ENST00000398667 ENST00000398666 ENST00000341305 ENST00000569741 ENST00000380147 ENST00000355235 ENST00000356834 ENST00000354329 ENST00000527372 ENST00000531253 ENST00000533112

Gene Information

Description Not Available

Gene Symbol:   TP53TG3B
Gene Name:   TP53 target 3B
Aliases:   MGC119888, TP53TG3, TP53TG3A, TP53TG3C, TP53TG3D
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000205457
Entrez:   729355
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.440000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download