PrimePCR™ SYBR® Green Assay: SAP25, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041067

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SAP25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SAP25, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038345
Assay Design:   exonic
Chromosome Location:   7:100169896-100169987question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000559107 ENST00000264245 ENST00000542088 ENST00000431360 ENST00000430784 ENST00000550217 ENST00000549374 ENST00000528759 ENST00000533655 ENST00000430323 ENST00000329143 ENST00000412330 ENST00000448296 ENST00000548614 ENST00000546637 ENST00000458640 ENST00000396172 ENST00000417556 ENST00000569674 ENST00000538735 ENST00000416863 ENST00000413678 ENST00000441425 ENST00000453138 ENST00000552288 ENST00000546633 ENST00000586262 ENST00000456476 ENST00000415689 ENST00000601144 ENST00000266659 ENST

Gene Information

Description Not Available

Gene Symbol:   SAP25
Gene Name:   SAP25
Aliases:   Not Available
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000205307
Entrez:   100316904
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download