PrimePCR™ SYBR® Green Assay: PRSS1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041066

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038344
Assay Design:   exonic
Chromosome Location:   7:142459625-142459741question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000567590 ENST00000561535 ENST00000570031 ENST00000486171 ENST00000311737 ENST00000492062

Gene Information

This gene encodes a trypsinogen which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq Jul 2008]

Gene Symbol:   PRSS1
Gene Name:   protease, serine, 1 (trypsin 1)
Aliases:   DKFZp779A0837, FLJ57296, MGC120175, MGC149362, TRP1, TRY1, TRY4, TRYP1
RefSeq:   NC_000007.13 NG_001333.2 NG_008307.2 NT_007914.15
Ensembl:   ENSG00000204983
Entrez:   5644
UniGene:   Hs.449281
Chromosome Mapping:   7q32-qter,7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.150000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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