PrimePCR™ SYBR® Green Assay: PSORS1C1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041057

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PSORS1C1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PSORS1C1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038335
Assay Design:   exonic
Chromosome Location:   6:31107591-31107732question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000441092 ENST00000382112 ENST00000382111 ENST00000400246 ENST00000382105 ENST00000373509 ENST00000433334 ENST00000420214 ENST00000259881 ENST00000547221 ENST00000481450 ENST00000448455 ENST00000342644 ENST00000372027 ENST00000310355 ENST00000270530 ENST00000376289 ENST00000549149 ENST00000344204 ENST00000509967 ENST00000513206 ENST00000537187

Gene Information

This gene is one of several genes thought to confer susceptibility to psoriasis and systemic sclerosis located on chromosome 6 near the major histocompatibility complex (MHC) class I region. [provided by RefSeq Sep 2011]

Gene Symbol:   PSORS1C1
Gene Name:   psoriasis susceptibility 1 candidate 1
Aliases:   C6orf16, MGC132530, SEEK1
RefSeq:   NC_000006.11 NG_012192.1 NG_021348.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204540
Entrez:   170679
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download