PrimePCR™ SYBR® Green Assay: NBPF24, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041040

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NBPF24, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NBPF24, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038318
Assay Design:   exonic
Chromosome Location:   1:147594369-147594477question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000370038 ENST00000415641 ENST00000495380 ENST00000531446 ENST00000444143 ENST00000370040 ENST00000294652 ENST00000369339 ENST00000448873 ENST00000342960 ENST00000491285 ENST00000429672 ENST00000479926 ENST00000339388 ENST00000369323 ENST00000442909 ENST00000446760 ENST00000309471 ENST00000369226 ENST00000369228 ENST00000369202 ENST00000369188 ENST00000369189 ENST00000414710 ENST00000583866 ENST00000579935 ENST00000580077 ENST00000581201

Gene Information

Description Not Available

Gene Symbol:   NBPF24
Gene Name:   neuroblastoma breakpoint family, member 24
Aliases:   DKFZp451B1418
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000203836
Entrez:   728912
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.230000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download