PrimePCR™ SYBR® Green Assay: CSAG1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041033

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CSAG1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CSAG1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038311
Assay Design:   exonic
Chromosome Location:   X:151909250-151909348question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000289272 ENST00000409494 ENST00000321773 ENST00000409012 ENST00000333046 ENST00000450791 ENST00000204726 ENST00000427522 ENST00000467148 ENST00000260408 ENST00000586582 ENST00000586965 ENST00000301293 ENST00000512062 ENST00000561792 ENST00000289175 ENST00000244815 ENST00000392000 ENST00000356805 ENST00000333896 ENST00000356443 ENST00000261606 ENST00000400569 ENST00000409522 ENST00000569479 ENST00000340940 ENST00000393039 ENST00000565624 ENST00000437464 ENST00000370287 ENST00000452779 ENST

Gene Information

This gene encodes a member of a family of tumor antigens. The protein is expressed in chondrosarcomas but may also be expressed in normal tissues such as testis. Alternative splicing of this gene results in two transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   CSAG1
Gene Name:   chondrosarcoma associated gene 1
Aliases:   CSAGE, CT24.1
RefSeq:   NC_000023.10 NG_015852.1 NT_167198.1 NG_013228.1
Ensembl:   ENSG00000198930
Entrez:   158511
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.950000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download