PrimePCR™ SYBR® Green Assay: FCGR1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041019

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FCGR1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FCGR1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038297
Assay Design:   exonic
Chromosome Location:   1:120930050-120930199question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000369384 ENST00000369383 ENST00000444948 ENST00000369168 ENST00000260364 ENST00000584115 ENST00000577333 ENST00000580679 ENST00000579737 ENST00000582600 ENST00000580698

Gene Information

Three distinct but closely related classes of receptors that bind the Fc portion of IgG have been identified (Fcgamma RI II and III). The FcgammaRI family consists of three closely related genes termed A B and C. This gene encodes the low affinity FcgammaRIB receptor that may play an important role in humoral immune response. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   FCGR1B
Gene Name:   Fc fragment of IgG, high affinity Ib, receptor (CD64)
Aliases:   CD64, IGFRB
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000198019
Entrez:   2210
Chromosome Mapping:   1p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.820000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download