PrimePCR™ SYBR® Green Assay: IL28B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041006

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IL28B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IL28B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038284
Assay Design:   exonic
Chromosome Location:   19:39735523-39735646question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000422132 ENST00000423807 ENST00000419060 ENST00000262715 ENST00000556935 ENST00000545983 ENST00000223095 ENST00000445463 ENST00000405694 ENST00000263776 ENST00000413851

Gene Information

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene interleukin 28A (IL28A) and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor beta (IL10RB) and interleukin 28 receptor alpha (IL28RA). [provided by RefSeq Jul 2008]

Gene Symbol:   IL28B
Gene Name:   interleukin 28B (interferon, lambda 3)
Aliases:   IFNL3, IL-28B, IL28C
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000197110
Entrez:   282617
Chromosome Mapping:   19q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download