PrimePCR™ SYBR® Green Assay: HBG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040999

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HBG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HBG2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038277
Assay Design:   exonic
Chromosome Location:   11:5274426-5274526question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000252891 ENST00000357947 ENST00000371890 ENST00000294337 ENST00000371891 ENST00000357641 ENST00000339717 ENST00000534694 ENST00000534400 ENST00000543502 ENST00000443324 ENST00000380252 ENST00000380259 ENST00000336906 ENST00000355962 ENST00000269228 ENST00000591051 ENST00000412552 ENST00000333640 ENST00000297661 ENST00000379186 ENST00000359544 ENST00000545103 ENST00000543944 ENST00000536252 ENST00000540348 ENST00000397910

Gene Information

The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq Jul 2008]

Gene Symbol:   HBG2
Gene Name:   hemoglobin, gamma G
Aliases:   FLJ76540
RefSeq:   NC_000011.9 NG_000007.3 NT_009237.18
Ensembl:   ENSG00000196565
Entrez:   3048
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.370000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download