PrimePCR™ SYBR® Green Assay: PTRH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040974

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038252
Assay Design:   exonic
Chromosome Location:   9:130476254-130476376question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000423807 ENST00000419060 ENST00000543175 ENST00000254853 ENST00000424747 ENST00000394105 ENST00000222390 ENST00000354283 ENST00000452510

Gene Information

Description Not Available

Gene Symbol:   PTRH1
Gene Name:   peptidyl-tRNA hydrolase 1 homolog (S. cerevisiae)
Aliases:   C9orf115, MGC51999, PTH1
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000187024
Entrez:   138428
UniGene:   Hs.653265
Chromosome Mapping:   9q34.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.090000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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