This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040971
PrimePCR™ PreAmp for SYBR® Green Assay: PRG2, Human
PrimePCR™ Template for SYBR® Green Assay: PRG2, Human
The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA) angiotensinogen (AGT) and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage exfoliation and bronchospasm in allergic diseases. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.