PrimePCR™ SYBR® Green Assay: DEFB119, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040932

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038210
Assay Design:   exonic
Chromosome Location:   20:29965024-29965160question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000373615 ENST00000446480 ENST00000378891 ENST00000378888 ENST00000306085 ENST00000368267 ENST00000332191 ENST00000339144 ENST00000376321

Gene Information

Description Not Available

Gene Symbol:   DEFB119
Gene Name:   defensin, beta 119
Aliases:   DEFB-19, DEFB-20, DEFB120, ESC42-RELA, ESC42-RELB, MGC71893
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000180483
Entrez:   245932
UniGene:   Hs.516998
Chromosome Mapping:   20q11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.450000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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