PrimePCR™ SYBR® Green Assay: FAHD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040929

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038207
Assay Design:   exonic
Chromosome Location:   16:1877634-1877826question
Amplicon Length:   163
Splice Variants Targeted:   ENST00000382668 ENST00000382666 ENST00000427358 ENST00000589117 ENST00000316433 ENST00000590588

Gene Information

Description Not Available

Gene Symbol:   FAHD1
Gene Name:   fumarylacetoacetate hydrolase domain containing 1
Aliases:   C16orf36, DKFZp566J2046, MGC74876, YISKL
RefSeq:   NC_000016.9 NG_023249.1 NT_010393.16
Ensembl:   ENSG00000180185
Entrez:   81889
UniGene:   Hs.513265
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.330000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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