PrimePCR™ SYBR® Green Assay: TOMM5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040914

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TOMM5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TOMM5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038192
Assay Design:   exonic
Chromosome Location:   9:37592452-37592568question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000523826 ENST00000339803 ENST00000379808 ENST00000552788 ENST00000549814 ENST00000524140 ENST00000341319 ENST00000558729 ENST00000341618 ENST00000399935 ENST00000399934 ENST00000399947 ENST00000339024 ENST00000399928 ENST00000286791 ENST00000545939 ENST00000300576 ENST00000434739 ENST00000361937 ENST00000401393 ENST00000377773 ENST00000321301 ENST00000401811 ENST00000544379 ENST00000453097 ENST00000421285 ENST00000279058 ENST00000417257 ENST00000402883 ENST00000290438 ENST00000356884 ENST

Gene Information

Description Not Available

Gene Symbol:   TOMM5
Gene Name:   translocase of outer mitochondrial membrane 5 homolog (yeast)
Aliases:   C9orf105, RP11-263I4.1, Tom5, bA613M10.3
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000175768
Entrez:   401505
Chromosome Mapping:   9p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 39.310000
Efficiency 86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download