PrimePCR™ SYBR® Green Assay: SF1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040863

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: SF1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SF1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038140
Assay Design:   exonic
Chromosome Location:   11:64535067-64535194question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000339591 ENST00000449880 ENST00000450894 ENST00000200181 ENST00000579662 ENST00000396219 ENST00000352665 ENST00000299764 ENST00000374846 ENST00000352456 ENST00000595018 ENST00000392006 ENST00000378215 ENST00000602130 ENST00000593587 ENST00000263367 ENST00000599614 ENST00000263275 ENST00000393313 ENST00000265827 ENST00000377387 ENST00000377390 ENST00000227503 ENST00000377394 ENST00000334944 ENST00000443908 ENST00000433274 ENST00000486867 ENST00000422298 ENST00000502732 ENST00000512653 ENST

Gene Information

This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq May 2010]

Gene Symbol:   SF1
Gene Name:   splicing factor 1
Aliases:   BBP, D11S636, MBBP, ZFM1, ZNF162
RefSeq:   NC_000011.9 NG_013018.1 NT_167190.1 NG_021399.1
Ensembl:   ENSG00000168066
Entrez:   7536
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.260000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download