PrimePCR™ SYBR® Green Assay: ZFYVE19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040854

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038131
Assay Design:   exonic
Chromosome Location:   15:41105536-41105930question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000570108 ENST00000564258 ENST00000355341 ENST00000336455 ENST00000299173 ENST00000566407

Gene Information

Description Not Available

Gene Symbol:   ZFYVE19
Gene Name:   zinc finger, FYVE domain containing 19
Aliases:   FLJ14840, MPFYVE
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000166140
Entrez:   84936
UniGene:   Hs.121676
Chromosome Mapping:   15q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.730000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download