PrimePCR™ SYBR® Green Assay: HSPA12A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040851

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HSPA12A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HSPA12A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038128
Assay Design:   exonic
Chromosome Location:   10:118464675-118464788question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000573124 ENST00000573484 ENST00000333480 ENST00000432126 ENST00000194118 ENST00000507481 ENST00000262301 ENST00000543238 ENST00000568897 ENST00000399843 ENST00000399002 ENST00000378194 ENST00000374323 ENST00000374321 ENST00000322157 ENST00000369209 ENST00000468935 ENST00000300146 ENST00000470794 ENST00000219271 ENST00000583692 ENST00000581986

Gene Information

Description Not Available

Gene Symbol:   HSPA12A
Gene Name:   heat shock 70kDa protein 12A
Aliases:   FLJ13874, KIAA0417
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000165868
Entrez:   259217
Chromosome Mapping:   10q26.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 36.460000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download