PrimePCR™ SYBR® Green Assay: SPRR2D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040840

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038117
Assay Design:   exonic
Chromosome Location:   1:153012366-153012501question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000320955 ENST00000231061 ENST00000539687 ENST00000522348 ENST00000360379 ENST00000368758 ENST00000368757 ENST00000368756

Gene Information

Description Not Available

Gene Symbol:   SPRR2D
Gene Name:   small proline-rich protein 2D
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163216
Entrez:   6703
UniGene:   Hs.505327
Chromosome Mapping:   1q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.740000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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