PrimePCR™ SYBR® Green Assay: ANKRD30BL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040839

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANKRD30BL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANKRD30BL, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038116
Assay Design:   exonic
Chromosome Location:   2:132914550-132914901question
Amplicon Length:   159
Splice Variants Targeted:   ENST00000325874 ENST00000395123 ENST00000395124 ENST00000352187 ENST00000529554 ENST00000533524 ENST00000374810 ENST00000374809 ENST00000358984 ENST00000447268 ENST00000268711 ENST00000395757 ENST00000355815 ENST00000261646 ENST00000338854 ENST00000335647 ENST00000335643 ENST00000367411 ENST00000358196 ENST00000593535 ENST00000244869 ENST00000409867 ENST00000594133 ENST00000576555

Gene Information

Description Not Available

Gene Symbol:   ANKRD30BL
Gene Name:   ankyrin repeat domain 30B-like
Aliases:   ANKRD30BP3, NCRNA00164
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000163046
Entrez:   554226
Chromosome Mapping:   2q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download