PrimePCR™ SYBR® Green Assay: PALM2-AKAP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040820

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PALM2-AKAP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PALM2-AKAP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038097
Assay Design:   exonic
Chromosome Location:   9:112898602-112898702question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000374530 ENST00000302798 ENST00000434623 ENST00000374525 ENST00000480388 ENST00000259318 ENST00000555236 ENST00000510514

Gene Information

PALM2-AKAP2 mRNAs are naturally occurring read-through products of the neighboring PALM2 and AKAP2 genes. The significance of these read-through mRNAs and the function the resulting fusion protein products have not yet been determined. Alternative splicing of this gene results in several transcript variants encoding different isoforms but the full-length nature of some of these variants has not been defined. [provided by RefSeq Oct 2010]

Gene Symbol:   PALM2-AKAP2
Gene Name:   PALM2-AKAP2 readthrough
Aliases:   AKAP2, DKFZp686H1948
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000157654
Entrez:   445815
Chromosome Mapping:   9q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.220000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download