PrimePCR™ SYBR® Green Assay: CCDC74B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040812

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CCDC74B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CCDC74B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038089
Assay Design:   exonic
Chromosome Location:   2:130902323-130902442question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000541478 ENST00000322349 ENST00000345598 ENST00000296452 ENST00000251047 ENST00000356392 ENST00000586836 ENST00000545100 ENST00000589042 ENST00000361874 ENST00000409438 ENST00000409240 ENST00000409868 ENST00000409567 ENST00000394003 ENST00000407639 ENST00000552551 ENST00000293308 ENST00000546897 ENST00000552150 ENST00000546826 ENST00000548998 ENST00000547413 ENST00000396505 ENST00000326932 ENST00000375587 ENST00000345988 ENST00000590782 ENST00000366699 ENST00000424347 ENST00000376598 ENST

Gene Information

Description Not Available

Gene Symbol:   CCDC74B
Gene Name:   coiled-coil domain containing 74B
Aliases:   DKFZp434E2321
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000152076
Entrez:   91409
Chromosome Mapping:   2q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.970000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download