PrimePCR™ SYBR® Green Assay: TRAF7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040756

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038033
Assay Design:   exonic
Chromosome Location:   16:2222192-2222477question
Amplicon Length:   165
Splice Variants Targeted:   ENST00000262304 ENST00000423118 ENST00000326181

Gene Information

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors such as TRAF7 are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family the TRAF domain which is involved in self-association and receptor binding.[supplied by OMIM Apr 2004]

Gene Symbol:   TRAF7
Gene Name:   TNF receptor-associated factor 7
Aliases:   DKFZp586I021, MGC7807, RFWD1, RNF119
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000131653
Entrez:   84231
UniGene:   Hs.713632
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.360000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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