This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040726
PrimePCR™ PreAmp for SYBR® Green Assay: OBP2A, Human
PrimePCR™ Template for SYBR® Green Assay: OBP2A, Human
This gene encodes a small extracellular protein belonging to the lipocalin superfamily. The protein is thought to transport small hydrophobic volatile molecules or odorants through the nasal mucus to olfactory receptors and may also function as a scavenger of highly concentrated or toxic odors. The protein is expressed as a monomer in the nasal mucus and can bind diverse types of odorants with a higher affinity for aldehydes and fatty acids. This gene and a highly similar family member are located in a cluster of lipocalin genes on chromosome 9. Alternatively spliced transcript variants have been described but their biological validity has not been determined. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.