PrimePCR™ SYBR® Green Assay: ZNF451, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040709

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037986
Assay Design:   exonic
Chromosome Location:   6:56963864-56989559question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000370733 ENST00000515290 ENST00000510483 ENST00000370706 ENST00000357489 ENST00000370708 ENST00000370702 ENST00000508603 ENST00000491832 ENST00000370710

Gene Information

Description Not Available

Gene Symbol:   ZNF451
Gene Name:   zinc finger protein 451
Aliases:   COASTER, FLJ23421, FLJ53246, FLJ90693, KIAA0576, KIAA1702, MGC26701, dJ417I1.1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000112200
Entrez:   26036
UniGene:   Hs.485628
Chromosome Mapping:   6p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.290000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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