PrimePCR™ SYBR® Green Assay: IFT20, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040703

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IFT20, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFT20, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037980
Assay Design:   exonic
Chromosome Location:   17:26658895-26659002question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000583970 ENST00000583880 ENST00000581127 ENST00000580696 ENST00000577872 ENST00000575742 ENST00000573233 ENST00000581681 ENST00000580505 ENST00000579419 ENST00000578009 ENST00000357896 ENST00000395418 ENST00000585089 ENST00000585313 ENST00000578122 ENST00000578985 ENST00000577498

Gene Information

Description Not Available

Gene Symbol:   IFT20
Gene Name:   intraflagellar transport 20 homolog (Chlamydomonas)
Aliases:   Not Available
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000109083
Entrez:   90410
Chromosome Mapping:   17q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.530000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download