PrimePCR™ SYBR® Green Assay: BCAT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040689

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037966
Assay Design:   exonic
Chromosome Location:   19:49300448-49300545question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000537152 ENST00000425898 ENST00000327787 ENST00000304874 ENST00000380839 ENST00000395332 ENST00000395331 ENST00000402551 ENST00000316273 ENST00000593515 ENST00000597011 ENST00000599246 ENST00000598162 ENST00000545387

Gene Information

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine isoleucine and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   BCAT2
Gene Name:   branched chain amino-acid transaminase 2, mitochondrial
Aliases:   BCAM, BCATM, BCT2, PP18
RefSeq:   NC_000019.9 NT_011109.16 NG_013003.1
Ensembl:   ENSG00000105552
Entrez:   587
UniGene:   Hs.512670
Chromosome Mapping:   19q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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