PrimePCR™ SYBR® Green Assay: HDAC10, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040680

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HDAC10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HDAC10, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037957
Assay Design:   exonic
Chromosome Location:   22:50688856-50688948question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000394078 ENST00000508305 ENST00000239440 ENST00000504448 ENST00000220496 ENST00000424267 ENST00000216271 ENST00000448072 ENST00000349505 ENST00000400237 ENST00000400236 ENST00000260283 ENST00000393452 ENST00000554180 ENST00000393454 ENST00000553617 ENST00000335725 ENST00000555880 ENST00000357382 ENST00000298622 ENST00000375482 ENST00000416701 ENST00000337352 ENST00000287078 ENST00000335494 ENST00000361170 ENST00000392056 ENST00000344657 ENST00000400767

Gene Information

The protein encoded by this gene belongs to the histone deacetylase family members of which deacetylate lysine residues on the N-terminal part of the core histones. Histone deacetylation modulates chromatin structure and plays an important role in transcriptional regulation cell cycle progression and developmental events. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   HDAC10
Gene Name:   histone deacetylase 10
Aliases:   DKFZp761B039, HD10, MGC149722
RefSeq:   NC_000022.10 NT_011526.7
Ensembl:   ENSG00000100429
Entrez:   83933
Chromosome Mapping:   22q13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.660000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download