PrimePCR™ SYBR® Green Assay: RNH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040644

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RNH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RNH1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037921
Assay Design:   exonic
Chromosome Location:   11:500502-500641question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000561891 ENST00000534797 ENST00000397615 ENST00000397604 ENST00000533410 ENST00000438658 ENST00000354420 ENST00000356187 ENST00000527485 ENST00000529368 ENST00000529306 ENST00000531149 ENST00000397614 ENST00000526712 ENST00000396398 ENST00000355265 ENST00000447274 ENST00000511455 ENST00000216122

Gene Information

Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al. 1988 [PubMed 3219362]). In addition to control of intracellular RNases the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor of 50000 Da binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.[supplied by OMIM Jul 2010]

Gene Symbol:   RNH1
Gene Name:   ribonuclease/angiogenin inhibitor 1
Aliases:   MGC18200, MGC4569, MGC54054, RAI, RNH
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000023191
Entrez:   6050
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.640000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download