This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040572
PrimePCR™ PreAmp for SYBR® Green Assay: HAX1, Human
PrimePCR™ Template for SYBR® Green Assay: HAX1, Human
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1 a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene mutations in which are associated with autosomal-dominant polycystic kidney disease and with the F-actin-binding protein cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria however recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.