PrimePCR™ SYBR® Green Assay: HAX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040572

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037849
Assay Design:   exonic
Chromosome Location:   1:154248168-154248264question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000328703 ENST00000457918 ENST00000483970 ENST00000532105

Gene Information

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1 a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene mutations in which are associated with autosomal-dominant polycystic kidney disease and with the F-actin-binding protein cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria however recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HAX1
Gene Name:   HCLS1 associated protein X-1
Aliases:   FLJ17042, FLJ18492, FLJ93803, HCLSBP1, HS1BP1, SCN3
RefSeq:   NC_000001.10 NT_004487.19 NG_007369.1
Ensembl:   ENSG00000143575
Entrez:   10456
UniGene:   Hs.199625
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.100000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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