This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040554
PrimePCR™ PreAmp for SYBR® Green Assay: HTR3D, Human
PrimePCR™ Template for SYBR® Green Assay: HTR3D, Human
The protein encoded this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit D of the type 3 receptor for 5-hydroxytryptamine (serotonin) a biogenic hormone that functions as a neurotransmitter a mitogen and a hormone. This hormone has been linked to neuropsychiatric disorders including anxiety depression and migraine. Serotonin receptors causes fast and depolarizing responses in neurons following activation. The genes encoding subunits C D and E of this type 3 receptor form a cluster on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.