PrimePCR™ SYBR® Green Assay: PDCD6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040552

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037829
Assay Design:   exonic
Chromosome Location:   5:314602-314695question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000264933 ENST00000505221 ENST00000507528 ENST00000507473

Gene Information

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor- Fas- and glucocorticoid-induced programmed cell death. In mice deficient for this gene product however apoptosis was not blocked suggesting this gene product is functionally redundant. [provided by RefSeq Jul 2008]

Gene Symbol:   PDCD6
Gene Name:   programmed cell death 6
Aliases:   ALG-2, FLJ46208, MGC111017, MGC119050, MGC9123, PEF1B
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000249915
Entrez:   10016
UniGene:   Hs.50823
Chromosome Mapping:   5p15.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.930000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download