PrimePCR™ SYBR® Green Assay: TMEM67, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040405

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037682
Assay Design:   exonic
Chromosome Location:   8:94767289-94768021question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000452276 ENST00000453321 ENST00000498673 ENST00000518319 ENST00000409623 ENST00000453906 ENST00000521517

Gene Information

The protein encoded by this gene localizes to the primary cilium and to the plasma membrane. The gene functions in centriole migration to the apical membrane and formation of the primary cilium. Multiple transcript variants encoding different isoforms have been found for this gene. Defects in this gene are a cause of Meckel syndrome type 3 (MKS3) and Joubert syndrome type 6 (JBTS6). [provided by RefSeq Nov 2008]

Gene Symbol:   TMEM67
Gene Name:   transmembrane protein 67
Aliases:   JBTS6, MECKELIN, MGC26979, MKS3, NPHP11, TNEM67
RefSeq:   NC_000008.10 NT_008046.16 NG_009190.1
Ensembl:   ENSG00000164953
Entrez:   91147
UniGene:   Hs.116240
Chromosome Mapping:   8q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997000
y-intercept 37.550000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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