PrimePCR™ SYBR® Green Assay: CHTOP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0040386

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CHTOP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CHTOP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037663
Assay Design:   exonic
Chromosome Location:   1:153610796-153610898question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000368694 ENST00000403433 ENST00000368687 ENST00000368686 ENST00000368690

Gene Information

This gene encodes a small nuclear protein that is characterized by a arginine and glycine rich region. The encoded protein may be involved in cell cycle progression. This protein interacts with protein arginine methyltransferases and plays a role in the activation of estradiol-dependent transcription. Alternate splicing results in multiple transcript variants. [provided by RefSeq Sep 2011]

Gene Symbol:   CHTOP
Gene Name:   chromatin target of PRMT1
Aliases:   C1orf77, DKFZp547E1010, FLJ40551, FOP, MGC131924, MGC86949, RP1-178F15.2, SRAG, pp7704
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000160679
Entrez:   26097
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 35.630000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download