PrimePCR™ SYBR® Green Assay: PPIAL4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040269

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPIAL4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPIAL4A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037546
Assay Design:   exonic
Chromosome Location:   1:147954784-147954915question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000355968 ENST00000489459 ENST00000468812 ENST00000419275 ENST00000540273 ENST00000539781 ENST00000544708 ENST00000369222 ENST00000581138 ENST00000581164 ENST00000584068 ENST00000583898 ENST00000577856 ENST00000585245 ENST00000583101

Gene Information

Description Not Available

Gene Symbol:   PPIAL4A
Gene Name:   peptidylprolyl isomerase A (cyclophilin A)-like 4A
Aliases:   COAS2, MGC163248, MGC163250, PPIAL4, PPIAL4B, PPIAL4C, PPIAL4D, PPIAL4G
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000255963
Entrez:   164022
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.550000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download