PrimePCR™ SYBR® Green Assay: REXO1L2P, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040268

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: REXO1L2P, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: REXO1L2P, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037545
Assay Design:   exonic
Chromosome Location:   8:86757695-86757798question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000393359 ENST00000258449 ENST00000170168 ENST00000233154 ENST00000393349 ENST00000295522 ENST00000260402 ENST00000557821 ENST00000456256 ENST00000400473 ENST00000256925 ENST00000420687 ENST00000361417 ENST00000356063 ENST00000527898 ENST00000524713 ENST00000400427 ENST00000400424 ENST00000408989 ENST00000408910 ENST00000310127 ENST00000525591 ENST00000539485 ENST00000379010 ENST00000425429 ENST00000540724 ENST00000379789 ENST00000317350 ENST00000379785 ENST00000360521 ENST00000379775 ENST

Gene Information

Description Not Available

Gene Symbol:   REXO1L2P
Gene Name:   REX1, RNA exonuclease 1 homolog (S. cerevisiae)-like 2, pseudogene
Aliases:   Not Available
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000255940
Entrez:   100288527
Chromosome Mapping:   8q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download