PrimePCR™ SYBR® Green Assay: LY6G6D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040239

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LY6G6D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LY6G6D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037516
Assay Design:   exonic
Chromosome Location:   6:31685376-31685468question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000519716 ENST00000428389 ENST00000252773 ENST00000395965 ENST00000556331 ENST00000414847 ENST00000434515 ENST00000430137 ENST00000557698 ENST00000439683 ENST00000440095 ENST00000456155 ENST00000556581 ENST00000503322 ENST00000375825 ENST00000375824 ENST00000455955 ENST00000444880 ENST00000439522 ENST00000554860 ENST00000428105 ENST00000420378 ENST00000421590 ENST00000430366 ENST00000419389 ENST00000445443 ENST00000383415 ENST00000400080 ENST00000400084

Gene Information

LY6G6D belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al. 2002 [PubMed 12079290]).[supplied by OMIM Apr 2009]

Gene Symbol:   LY6G6D
Gene Name:   lymphocyte antigen 6 complex, locus G6D
Aliases:   C6orf23, G6D, LY6-D, MEGT1, MGC150334, NG25
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000244355
Entrez:   58530
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.230000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download