PrimePCR™ SYBR® Green Assay: PPT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040136

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037413
Assay Design:   exonic
Chromosome Location:   6:32122807-32123467question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000447703 ENST00000423347 ENST00000440507 ENST00000424353 ENST00000452304 ENST00000426325 ENST00000548089 ENST00000551222 ENST00000458042 ENST00000427896 ENST00000429189 ENST00000467040 ENST00000424667 ENST00000425659 ENST00000478621 ENST00000415972 ENST00000416892 ENST00000412651 ENST00000457509 ENST00000440533 ENST00000445462 ENST00000451460 ENST00000547565 ENST00000550796 ENST00000458119 ENST00000383152 ENST00000399665 ENST00000399662 ENST00000399660 ENST00000433748 ENST00000551703 ENST

Gene Information

This gene encodes a member of the palmitoyl-protein thioesterase family. The encoded glycosylated lysosomal protein has palmitoyl-CoA hydrolase activity in vitro but does not hydrolyze palmitate from cysteine residues in proteins. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream EGFL8 (EGF-like-domain multiple 8) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   PPT2
Gene Name:   palmitoyl-protein thioesterase 2
Aliases:   C6orf8, DKFZp564P1516, G14, PPT-2
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000221988
Entrez:   9374
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download