PrimePCR™ SYBR® Green Assay: ZNF321P, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040134

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037411
Assay Design:   exonic
Chromosome Location:   19:53432336-53432485question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000421239 ENST00000601120 ENST00000359798 ENST00000465448 ENST00000486474 ENST00000461321 ENST00000391785 ENST00000301093 ENST00000540331 ENST00000602162 ENST00000319783 ENST00000595798 ENST00000601643 ENST00000543227 ENST00000540744 ENST00000453741 ENST00000338230 ENST00000438150 ENST00000457749 ENST00000391783 ENST00000596559 ENST00000594602 ENST00000360272 ENST00000414252 ENST00000595646 ENST00000243639 ENST00000396409 ENST00000390651 ENST00000357666 ENST00000444460 ENST00000434371 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF321P
Gene Name:   zinc finger protein 321, pseudogene
Aliases:   DKFZp781C2298, FLJ39509, MGC35402, ZNF321
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000221874
Entrez:   399669
UniGene:   Hs.675715
Chromosome Mapping:   19q13.41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.220000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download